Gain reproducible insight into vesicle behaviour in solution. Detect aggregation, understand heterogeneity and optimise formulations to accelerate extracellular vesicle therapeutic development with Zetasizer.
Improve Exosome and Extracellular Vesicle Characterisation with Zetasizer Dynamic Light Scattering, MADLS and Zeta Potential Analysis for Confident Particle Characterisation and Formulation Stability.
Exosomes and extracellular vesicles (EVs) are playing an increasingly important role in biologics research, supporting applications ranging from biomarker discovery and diagnostics to drug delivery, regenerative medicine and cell therapy. Their nanoscale size and naturally heterogeneous composition make them particularly challenging to characterise consistently. Small changes in isolation methods, formulation conditions or storage can alter particle behaviour, affecting biological activity, manufacturing consistency and confidence in research outcomes.
Many analytical techniques only reveal instability once significant changes have already occurred, limiting opportunities to optimise formulations before valuable samples or development time are lost. Zetasizer provides earlier insight into particle behaviour, enabling scientists to detect subtle changes before they become critical quality issues.
Zetasizer helps you stay ahead of instability, giving you the insight needed to optimise exosome and extracellular vesicle characterisation with confidence.
With Zetasizer you can:
✔ Detect aggregation earlier
✔ Improve formulation stability
✔ Increase confidence in development decisions
✔ Generate highly reproducible, trusted data
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Even mature extracellular vesicle workflows can contain hidden particle-related risks that reduce confidence in analytical results and downstream development. Because EV samples are highly sensitive to purification methods, formulation conditions and storage environments, small changes can significantly influence particle behaviour without immediately becoming apparent.
These issues often emerge during scale-up, technology transfer or commercial manufacturing, when process deviations become significantly more expensive to correct.
Earlier insight into particle behaviour can make all the difference.
Subtle changes in vesicle size, surface charge and interaction behaviour can significantly impact delivery efficiency, targeting accuracy and overall therapeutic performance. These instabilities are often missed during early development and only become apparent under real-world conditions such as scale-up, storage or transport.
Zetasizer is designed to uncover these risks earlier in development.
Using highly sensitive dynamic light scattering and zeta potential analysis, Zetasizer detects even small changes in vesicle size distribution and surface charge. This enables scientists to identify early-stage aggregation, instability and interaction changes before they impact performance. The result is faster, more informed decision-making and reduced risk of late-stage failure.
Aggregation is often the first indication that extracellular vesicle formulations are beginning to lose stability. Even relatively small increases in aggregate formation can influence biological function, therapeutic performance, biomarker interpretation and manufacturing consistency. Conventional analytical methods frequently detect these changes only after instability has progressed, leaving fewer opportunities to optimise formulations before valuable samples are affected.
Dynamic Light Scattering enables highly sensitive particle size measurements that reveal subtle changes in particle populations long before visible aggregation occurs. Early detection allows scientists to compare formulations more effectively, evaluate process changes with greater confidence and make informed development decisions supported by reliable analytical data.
Adaptive Correlation enhances this capability by improving measurement sensitivity in noisy, unstable, highly concentrated or broad particle size distributions that are commonly encountered during extracellular vesicle research. Improved correlation quality delivers more robust data from challenging biological samples while reducing uncertainty in complex analyses.
With Zetasizer you can:
✔ Detect aggregation before biological performance is compromised.
✔ Compare formulations with greater analytical confidence.
✔ Reduce development risk through earlier particle characterisation.
Early insight into aggregation allows researchers to optimise purification strategies, refine formulations and maintain confidence throughout development, reducing the likelihood of costly delays later in the product lifecycle.
Maintaining formulation stability is essential throughout extracellular vesicle development, from laboratory research through to manufacturing and storage. Small changes in buffer composition, ionic strength, pH or excipient selection can influence aggregation behaviour, shelf life and therapeutic efficacy. Understanding these interactions early enables development teams to build more robust formulations while minimising the risk of instability during scale-up.
Zeta Potential provides valuable insight into particle surface charge, allowing scientists to predict colloidal stability, assess aggregation risk and identify formulation stability windows. Measuring surface charge also supports comparisons between formulations, helping researchers understand how seemingly minor formulation adjustments influence long-term product performance.
These insights enable evidence-based decisions throughout formulation development, reducing reliance on trial-and-error approaches while improving confidence in manufacturing robustness and product consistency.
With Zetasizer you can:
✔ Improve formulation robustness and long-term stability.
✔ Predict aggregation risk before instability develops.
✔ Support confident formulation optimisation throughout development.
Understanding the relationship between surface charge and colloidal stability enables scientists to design formulations that are better suited to manufacturing, transportation and long-term storage, improving both product quality and development efficiency.
Extracellular vesicle samples rarely consist of a single, uniform particle population. Instead, they often contain multiple vesicle subpopulations alongside proteins, lipoproteins and other nanoparticles that make accurate characterisation increasingly difficult. Understanding these complex particle distributions is essential when evaluating purification methods, formulation changes or manufacturing processes.
MADLS enhances conventional Dynamic Light Scattering by combining measurements from multiple detection angles to improve particle population resolution. This approach enables clearer separation of multiple particle populations, greater confidence when analysing broad size distributions and more robust measurements across varying sample concentrations.
The additional analytical detail provided by MADLS allows scientists to better understand heterogeneous extracellular vesicle preparations, supporting improved process optimisation and stronger confidence in development decisions.
With Zetasizer you can:
✔ Resolve complex particle populations with greater confidence.
✔ Improve characterisation of heterogeneous extracellular vesicle samples.
✔ Generate richer analytical insight throughout biologics development.
By delivering greater visibility into complex particle behaviour, MADLS helps researchers make more informed decisions while improving reproducibility across extracellular vesicle characterisation workflows.
Automated workflows powered by the Sample Assistant bring greater consistency and control to extracellular vesicle analysis. By standardising sample handling, measurement and data collection, the system reduces operator variability and ensures that each sample is analysed under the same conditions.
This is particularly important for EV systems, where small differences in preparation or measurement can significantly influence results. Consistent workflows ensure that subtle changes in vesicle behaviour are detected reliably and can be compared across experiments.
Designed for high-throughput environments, the Sample Assistant enables efficient screening of formulation conditions, batch comparisons and stability studies. Dynamic light scattering and zeta potential measurements remain robust, while automation improves reproducibility and data quality.
With the Sample Assistant you can:
✔ Standardise analytical methods across laboratories.
✔ Reduce user variability.
✔ Accelerate training.
✔ Deliver consistent, reproducible results every time.
By combining intelligent workflow guidance with powerful particle characterisation technologies, Sample Assistant helps laboratories improve efficiency while ensuring analytical confidence remains consistent across every measurement.
Understanding particle behaviour at every stage of extracellular vesicle development is essential for producing reproducible, high-quality biologics. By combining Dynamic Light Scattering, Adaptive Correlation, MADLS and Zeta Potential analysis within a single platform, Zetasizer provides the analytical confidence needed to detect instability earlier, optimise formulations and reduce development risk.
With Zetasizer you can:
Whether you are advancing extracellular vesicle therapeutics, investigating novel biomarkers or optimising manufacturing processes, Zetasizer provides the trusted analytical insight needed to accelerate development, strengthen scientific confidence and deliver more reliable outcomes throughout the biologics development lifecycle.
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The basics of Dynamic Light Scattering and electrophoretic light scattering will be discussed. I will review the Dynamic Light Scattering acronyms, i.e what is meant by NIBS and MADLS.
ZetaClass is a comprehensive webinar series on dynamic and electrophoretic light scattering where our experts walk you through the basic measuring principles, data interpretation, and tests performed to gauge data quality.
Join us as we unveil the new and expanded Zetasizer range: Zetasizer Advance. Dr Mike Kaszuba will introduce the new range and help you to pinpoint which system best fits your particle characterization requirements.
This webinar discusses some basic fundamentals of light scattering science and also common misconceptions about light scattering methods
In this webinar, you will learn about developing reliable and effective methods for dynamic light scattering (DLS) and electrophoretic light scattering (ELS) measurements using the Zetasizer Advance instrument. The presentation is geared toward users of DLS and ELS instruments who want to deepen their understanding of method development.
This webinar will explore how the Dynamic Light Scattering (DLS) technique, utilized by the Zetasizer Advance series, ensures compliance with United States Pharmacopeia (USP) guidelines, specifically USP 430.
This webinar will discuss each of the sections contained in United States Pharmacopeia (USP) 432 and what it means for users of Malvern Panalytical’s Zetasizer range of instruments who measure zeta potential in their workflow.
Join us to learn how to assess the quality of your data collected on the Zetasizer Advance instrument. Together we will go through real examples of data from particle size measurements that use Dynamic Light Scattering (DLS) and zeta potential measurements obtained with Electrophoretic Light Scattering (ELS) assessing data quality and sharing tips on how to improve confidence in the results.